No. Kenji will not unfollow anyone you were following before you connected your account. Those connections are automatically protected by the Auto-Whitelist feature, so you don’t have to manually add them. Your existing followings (friends, collaborators, clients) stay untouched.
What About Users I Follow Manually?
After connecting your account, any new users you follow manually are not automatically whitelisted. This also applies to users who followed you back as part of Kenji’s growth system. If you want to keep certain users from being unfollowed, make sure to add them to your whitelist manually.
Disclaimer: Instagram caps the number of accounts you can follow at 7,500. If you’re already following more than 6,500, you might run into limits that reduce how effectively Kenji can operate.